c Cell proliferation after Cullin7 overexpression in cells was measured using MTT assays. b The transfection efficiency of Cullin7 was analyzed by measuring transcript levels using qRT-PCR analyses in HepG2 cells. a Ectopic expression of Cullin7 in HepG2 cells analyzed by western blotting. Upregulation of Cullin7 enhances the proliferation, migration, and invasion capacities of HCC cells. Our studies provide evidence that overexpression of Cullin7 plays an important role in the pathogenesis and progression of hepatocellular carcinoma and may be a valuable marker for hepatocellular carcinoma management.Д¬ullin7 Hepatocellular carcinoma Invasion Proliferation Western blot. Conversely, knocking down Cullin7 expression with short hairpin RNAi in hepatocellular carcinoma HepG2 cells inhibited cell proliferation, growth, migration and invasion. Furthermore, by overexpressing Cullin7 in hepatocellular carcinoma HepG2 cells, we revealed that Cullin7 could significantly enhance cell proliferation, growth, migration and invasion. Statistical analysis found that overexpression of Cullin7 was significantly associated with lymph node metastasis, tumor thrombus of the portal vein and advanced clinical stage (P < 0.05). The immunohistochemistry analysis revealed that overexpression of Cullin7 occurred in 69.1% of hepatocellular carcinoma samples, which was a significantly higher rate than that in adjacent normal hepatic tissue (P < 0.01). We found elevated expression of both mRNA and protein levels of Cullin7 in hepatocellular carcinoma cell lines, and Cullin7 protein was significantly upregulated in hepatocellular carcinoma compared with paired normal hepatic tissues. In addition, the protein expression of Cullin7 was examined in 162 cases of archived hepatocellular carcinoma using immunohistochemistry. In the present study, the expression of Cullin7 in hepatocellular carcinoma cell lines and five surgical hepatocellular carcinoma specimens was detected with quantitative reverse transcription PCR and western blotting.

The aim of this study was to investigate the role of Cullin7 in pathogenesis and the progression of hepatocellular carcinoma. However, the part of Cullin7 in hepatocellular carcinoma remains unclear. Overexpression of Cullin7 is associated with some types of malignancies.

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